IL-2-dependent generation of natural killer cells from bone marrow: role of MAC-1-, NK1-1- precursors

Cell Immunol. 1992 May;141(2):323-31. doi: 10.1016/0008-8749(92)90151-e.

Abstract

We have previously shown that interleukin-2 (IL-2) is able to induce the generation of natural killer (NK) activity in bone marrow (BM) cell cultures from mice pretreated with 5-fluorouracil (5-FU). Cell fractionation experiments to analyze the nature of BM precursors indicate that MAC-1-, NK1-1- noncytotoxic precursors are induced by IL-2 to proliferate and generate cytolytic NK cells. These data demonstrate that the phenotype and functional characteristics of the IL-2-responsive cells in the FUBM are different from those of mature NK cells in that they are MAC-1+, NK1.1+, CD3- and susceptible to boosting by IFN-alpha.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens / analysis
  • Antigens, Differentiation, T-Lymphocyte / analysis
  • Antigens, Ly
  • Antigens, Surface
  • Bone Marrow / drug effects*
  • Bone Marrow Cells
  • CD3 Complex
  • Cell Differentiation
  • Female
  • Hematopoietic Stem Cells / immunology*
  • Interleukin-2 / pharmacology*
  • Killer Cells, Natural / immunology*
  • Lectins, C-Type
  • Macrophage-1 Antigen / analysis
  • Male
  • Mice
  • NK Cell Lectin-Like Receptor Subfamily B
  • Proteins / analysis
  • Receptors, Antigen, T-Cell / analysis
  • Tumor Cells, Cultured

Substances

  • Antigens
  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Ly
  • Antigens, Surface
  • CD3 Complex
  • Interleukin-2
  • Klrb1c protein, mouse
  • Lectins, C-Type
  • Macrophage-1 Antigen
  • NK Cell Lectin-Like Receptor Subfamily B
  • Proteins
  • Receptors, Antigen, T-Cell