Rapid synthesis of [18F]FDG without an evaporation step using an ionic liquid

Appl Radiat Isot. 2004 Dec;61(6):1241-6. doi: 10.1016/j.apradiso.2004.02.027.

Abstract

In this study, we describe a new 2-[18F]fluoro-2-deoxy-d-glucose ([18F]FDG) synthesis without a distillation step. This involves fluorinating in an ionic liquid-containing medium. A test for the effective elution of [18F]fluoride from the anion exchange resin showed the proper selection of the base and the required eluant composition, which is an essential requirement for the automation of [18F]FDG synthesis. An 18F-labeling study by nucleophilic substitution showed that the major factors controlling the yield were the temperature and the reaction medium composition. The 18F-fluorination proceeded with a labeling efficiency of 74.6+/-7.4% (n=8) for optimized conditions. Alkaline hydrolysis and purification carried out in the liquid phase provided a final decay-corrected [18F]FDG yield of 59.1+/-5.1% (n=3), a radiochemical purity of 91.9+/-3.7% (n=3), and a reaction time of 13 min. Alkaline hydrolysis and purification carried out in the solid phase provided a final decay-corrected [18F]FDG yield of 48.8+/-6.0% (n=3), a radiochemical purity of 96.0+/-4% (n=3), and a reaction time of 19 min. The rapid and straightforward synthesis of [18F]FDG can be achieved by eliminating all evaporation steps, which is made possible by the use of ionic liquid-containing media for the fluorination step.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Ion Exchange / methods*
  • Fluorodeoxyglucose F18 / chemical synthesis*
  • Fluorodeoxyglucose F18 / isolation & purification*
  • Ions
  • Isotope Labeling / methods*
  • Radiopharmaceuticals / chemical synthesis
  • Radiopharmaceuticals / isolation & purification
  • Solutions
  • Volatilization

Substances

  • Ions
  • Radiopharmaceuticals
  • Solutions
  • Fluorodeoxyglucose F18