Detection of catheter-related bloodstream infections by the differential-time-to-positivity method and gram stain-acridine orange leukocyte cytospin test in neutropenic patients after hematopoietic stem cell transplantation

J Clin Microbiol. 2004 Oct;42(10):4835-7. doi: 10.1128/JCM.42.10.4835-4837.2004.

Abstract

For febrile neutropenic patients who received hematopoietic stem cell transplantation, the Gram stain-acridine orange leukocyte cytospin (AOLC) test and the differential-time-to-positivity method (DTP) were performed. As a diagnostic tool for catheter-related bloodstream infections in these patients, the Gram stain-AOLC test has a lower sensitivity than does the DTP method but acceptable positive and negative predictive values.

MeSH terms

  • Acridine Orange
  • Bacteremia / diagnosis*
  • Bacteremia / microbiology
  • Bacteriological Techniques
  • Catheterization, Central Venous / adverse effects*
  • Catheters, Indwelling / adverse effects*
  • Equipment Contamination
  • Fluorescent Dyes
  • Gentian Violet
  • Hematopoietic Stem Cell Transplantation / adverse effects*
  • Humans
  • Leukocytes
  • Neutropenia / therapy*
  • Phenazines
  • Staining and Labeling
  • Time Factors

Substances

  • Fluorescent Dyes
  • Gram's stain
  • Phenazines
  • Acridine Orange
  • Gentian Violet