Expression profiling reveals a distinct transcription signature in follicular thyroid carcinomas with a PAX8-PPAR(gamma) fusion oncogene

Oncogene. 2005 Feb 17;24(8):1467-76. doi: 10.1038/sj.onc.1208135.

Abstract

The demonstration of the PAX8-PPAR(gamma) fusion oncogene in a subset of follicular thyroid tumors provides a new and promising starting point to dissect the molecular genetic events involved in the development of this tumor form. In the present study, we compared the gene expression profiles of follicular thyroid carcinomas (FTCs) bearing a PAX8-PPAR(gamma) fusion against FTCs that lack this fusion. Using unsupervised clustering and multidimensional scaling analyses, we show that FTCs possessing a PAX8-PPAR(gamma) fusion have a highly uniform and distinct gene expression signature that clearly distinguishes them from FTCs without the fusion. The PAX8-PPAR(gamma)(+) FTCs grouped in a defined cluster, where highly ranked genes were mostly associated with signal transduction, cell growth and translation control. Notably, a large number of ribosomal protein and translation-associated genes were concurrently underexpressed in the FTCs with the fusion. Taken together, our findings further support that follicular carcinomas with a PAX8-PPAR(gamma) rearrangement constitute a distinct biological entity. The current data represent one step to elucidate the molecular pathways in the development of FTCs with the specific PAX8-PPAR(gamma) fusion.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma, Follicular / diagnosis
  • Adenocarcinoma, Follicular / genetics*
  • Alcohol Oxidoreductases
  • Co-Repressor Proteins
  • Computational Biology
  • DNA-Binding Proteins / genetics
  • Diagnosis, Differential
  • Down-Regulation / genetics
  • Eye Proteins / genetics
  • Gene Expression Profiling
  • Humans
  • Nerve Tissue Proteins
  • Nuclear Proteins / genetics
  • Oligonucleotide Array Sequence Analysis
  • Oncogene Proteins, Fusion / biosynthesis
  • Oncogene Proteins, Fusion / genetics*
  • PAX8 Transcription Factor
  • PPAR gamma / genetics
  • Paired Box Transcription Factors
  • Protein Biosynthesis / genetics
  • Ribosomal Proteins / genetics
  • Thyroid Neoplasms / diagnosis
  • Thyroid Neoplasms / genetics*
  • Trans-Activators / genetics
  • Transcription, Genetic
  • Up-Regulation / genetics

Substances

  • Co-Repressor Proteins
  • DNA-Binding Proteins
  • Eye Proteins
  • Nerve Tissue Proteins
  • Nuclear Proteins
  • Oncogene Proteins, Fusion
  • PAX8 Transcription Factor
  • PAX8 protein, human
  • PPAR gamma
  • Paired Box Transcription Factors
  • Ribosomal Proteins
  • Trans-Activators
  • Alcohol Oxidoreductases
  • CTBP2 protein, human