Androgen receptor coregulator ARA267-alpha interacts with death receptor-6 revealed by the yeast two-hybrid

Sci China C Life Sci. 2004 Oct;47(5):442-8. doi: 10.1360/03yc0214.

Abstract

ARA267-alpha is a newly identified androgen receptor coactivator. In order to further elucidate its precise role in cells, using the ARA267-alpha fragment containing four PHD and one SET conserved domains as bait we revealed an ARA267-alpha-PHD-SET-interacting protein, death receptor-6 (DR6), in the yeast two-hybrid screening. DR6 is the member of TNF receptor family and has a death domain in its intracellular cytoplasmic portion (DR6cp) to mediate the cell apoptosis. The interaction between ARA267-alpha-PHD-SET and DR6cp was confirmed in vitro and in vivo. Our finding implied that androgen signaling pathway might cross talk with apoptosis signaling pathway through the interaction between ARA267-alpha and DR6.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Cell Line, Tumor
  • Cloning, Molecular
  • DNA, Complementary / metabolism
  • Escherichia coli / metabolism
  • Histone Methyltransferases
  • Histone-Lysine N-Methyltransferase
  • Humans
  • Immunoprecipitation
  • Intracellular Signaling Peptides and Proteins / metabolism
  • Intracellular Signaling Peptides and Proteins / physiology*
  • Mice
  • Nuclear Proteins
  • Plasmids / metabolism
  • Protein Binding
  • Protein Structure, Tertiary
  • Receptors, Androgen / chemistry*
  • Receptors, Androgen / metabolism
  • Receptors, Tumor Necrosis Factor / metabolism*
  • Signal Transduction
  • Two-Hybrid System Techniques

Substances

  • DNA, Complementary
  • Intracellular Signaling Peptides and Proteins
  • Nuclear Proteins
  • Receptors, Androgen
  • Receptors, Tumor Necrosis Factor
  • Tnfrsf21 protein, mouse
  • Histone Methyltransferases
  • Histone-Lysine N-Methyltransferase
  • NSD1 protein, human