Detection of human influenza A viruses by loop-mediated isothermal amplification

J Clin Microbiol. 2005 Jan;43(1):427-30. doi: 10.1128/JCM.43.1.427-430.2005.

Abstract

Here we describe the use of the loop-mediated isothermal amplification (LAMP) method to detect human influenza viruses (H1 to H3). Our results were correlated 100% with results deduced from routine clinical diagnostic tests. In addition, we also developed a LAMP assay specific for human beta-actin cDNA as a quality control test.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actins / genetics
  • DNA Primers
  • DNA, Complementary / analysis
  • DNA, Viral / analysis
  • Humans
  • Influenza A virus / genetics
  • Influenza A virus / isolation & purification*
  • Influenza, Human / virology
  • Nucleic Acid Amplification Techniques / methods*
  • Sensitivity and Specificity

Substances

  • Actins
  • DNA Primers
  • DNA, Complementary
  • DNA, Viral