Abstract
HIV-1 NEF genes were isolated directly from peripheral blood lymphocyte DNA of two HIV-1-infected individuals and cloned into an HXB-2-infectious molecular clone. The effect of NEF on virus production in T-cell lines and primary human lymphocytes was studied. Naturally occurring NEF accelerates virus production in primary human lymphocytes, but not in T-cell lines.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cells, Cultured
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Cloning, Molecular
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DNA, Viral
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Gene Products, nef / chemistry
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Gene Products, nef / genetics
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Genes, nef*
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HIV Long Terminal Repeat
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HIV-1 / genetics*
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HIV-1 / physiology
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Humans
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Kinetics
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Lymphocytes / microbiology
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Molecular Sequence Data
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Restriction Mapping
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Sequence Alignment
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T-Lymphocytes / microbiology
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Virus Replication / genetics
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nef Gene Products, Human Immunodeficiency Virus
Substances
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DNA, Viral
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Gene Products, nef
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nef Gene Products, Human Immunodeficiency Virus
Associated data
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GENBANK/M84627
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GENBANK/M84628
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GENBANK/M84629
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GENBANK/M84630
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GENBANK/M84631
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GENBANK/M84632