Somatostatin analogs in vitro effects in a growth hormone-releasing hormone-secreting bronchial carcinoid

J Clin Endocrinol Metab. 2005 Apr;90(4):2104-9. doi: 10.1210/jc.2004-2156. Epub 2005 Jan 25.

Abstract

A 29-yr-old woman presented with acromegaly, pituitary gland enlargement, and an isolated pulmonary mass of 3.3 cm in diameter, which displayed a very high tracer uptake after OctreoScan. Plasma GHRH levels were markedly elevated. The patient underwent left lung upper lobectomy, and histopathology disclosed a bronchial atypical carcinoid. The tissue was examined for somatostatin (SRIH) receptor subtypes (SSTRs) 1-5 expression by RT-PCR. Cultured tumor cells were treated with SRIH, lanreotide (BIM-23014), or SRIH analogs selective for SSTR2 (BIM-23120), SSTR5 (BIM-23206), or SSTR1 (BIM-23926). GHRH was measured in the medium after 6 h, and cell viability was assessed after 48 h. RT-PCR analysis showed expression of SSTR1, -2, and -5. GHRH secretion was significantly reduced by SRIH (-50%), Lan (-35%), as well as by the SSTR2, SSTR5, and SSTR1 selective agonists (-55, -75, and -20%, respectively), whereas cell viability was not affected. Our data show SSTR expression in a GHRH-secreting bronchial carcinoid and provide evidence that, in vitro, selective SSTR activation differently inhibit ectopic GHRH secretion. These findings suggest that SSTR-specific SRIH analogs may be useful in the medical therapy of GHRH-secreting bronchial carcinoids.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Bronchial Neoplasms / drug therapy*
  • Bronchial Neoplasms / metabolism
  • Bronchial Neoplasms / pathology
  • Carcinoid Tumor / drug therapy*
  • Carcinoid Tumor / metabolism
  • Carcinoid Tumor / pathology
  • Cell Survival / drug effects
  • Female
  • Growth Hormone-Releasing Hormone / metabolism*
  • Humans
  • Receptors, Somatostatin / agonists*
  • Receptors, Somatostatin / classification

Substances

  • Receptors, Somatostatin
  • Growth Hormone-Releasing Hormone