Comparative evaluation of RAST and MAST-CLA for six allergens for the diagnosis of inhalant allergic disease in 232 patients

Clin Exp Allergy. 1992 Feb;22(2):227-31. doi: 10.1111/j.1365-2222.1992.tb03077.x.

Abstract

The aim of this study was to compare a recent multiple allergosorbent chemiluminescent assay (MAST-CLA) with the RAST for the diagnosis of inhalant allergic disease in 232 patients with rhinitis and/or bronchial asthma. As judged by concordance of clinical history, skin prick tests to a range of six allergens common to our geographic area, and by nasal provocation tests, 70 patients were non-allergic and 162 allergic: 70 to grasses, 46 to mites, four to mugwort, eight to pellitory, and 34 were sensitive to several allergens. In our patient sample that, among other things, comprises subjects with fairly rare monoallergies, MAST-CLA testing gave results which closely corresponded to positive RAST for the allergens studied, and demonstrated a close correlation with the diagnosis of inhalant-specific allergy. Our results showed that, for overall allergens, MAST-CLA was lightly less sensitive and more specific than RAST (the two in vitro tests gave an identical overall efficiency).

Publication types

  • Comparative Study

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Allergens / analysis*
  • Animals
  • Child
  • Female
  • Humans
  • Immunosorbent Techniques*
  • Luminescent Measurements
  • Male
  • Middle Aged
  • Mites / immunology
  • Plants / immunology
  • Pollen / immunology
  • Radioallergosorbent Test / standards*
  • Respiratory Hypersensitivity / diagnosis*
  • Sensitivity and Specificity

Substances

  • Allergens