Evaluation of the 111In-(Sn)-citrate method to label antibodies for radioimmunotargeting studies

Int J Rad Appl Instrum B. 1992 Jan;19(1):21-5. doi: 10.1016/0883-2897(92)90180-7.

Abstract

Monoclonal antibody against carcinoembryonic antigen (CEA) was labeled by the 111In-(Sn)-citrate technique and its characteristics were determined from the point of radioimmunotargeting studies. Antibodies of high specific radioactivity could be labeled with a high labeling yield of greater than 90% without any further purification step, and without loss of immunoreactivity. In biodistribution studies using nude mice bearing CEA-producing tumors, an extremely high tumor-to-blood ratio (40 on day 6) was obtained since 111In in the blood cleared very rapidly, but nonspecific 111In localized in the liver and spleen should be seriously considered.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal*
  • Binding, Competitive
  • Carcinoembryonic Antigen / analysis
  • Carcinoembryonic Antigen / immunology
  • Chromatography, High Pressure Liquid
  • Citrates / chemistry*
  • Citric Acid
  • Indium Radioisotopes / chemistry*
  • Mice
  • Mice, Nude
  • Neoplasm Transplantation
  • Radioimmunodetection / methods*
  • Tin / chemistry*
  • Tin Compounds*
  • Tissue Distribution

Substances

  • Antibodies, Monoclonal
  • Carcinoembryonic Antigen
  • Citrates
  • Indium Radioisotopes
  • Tin Compounds
  • stannous chloride
  • Citric Acid
  • Tin