A test electron beam is propagated in a specially designed traveling wave tube. It interacts with a nonresonant wave, and its energy distribution is recorded at the tube output. We report the direct experimental observation of the spatially periodic electron velocity bunching, and of a nonlinear effect on the electron velocity modulation: the synchronization of the particles with the wave responsible for Landau damping in plasma physics. The results are explained by second order perturbation theory in the wave amplitude.