Optical probes for biological applications based on surface-enhanced Raman scattering from indocyanine green on gold nanoparticles

Anal Chem. 2005 Apr 15;77(8):2381-5. doi: 10.1021/ac050109v.

Abstract

We report surface-enhanced Raman scattering (SERS) studies on indocyanine green (ICG) on colloidal silver and gold and demonstrate a novel optical probe for applications in living cells. In addition to its own detection by the characteristic ICG SERS signatures, the ICG gold nanoprobe delivers spatially localized chemical information from its biological environment by employing SERS in the local optical fields of the gold nanoparticles. The probe offers the potential to increase the spectral specificity and selectivity of current chemical characterization approaches of living cells and biomaterials based on vibrational information.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Carcinoma / pathology
  • Gold Colloid / pharmacokinetics
  • Indocyanine Green / chemistry*
  • Male
  • Metal Nanoparticles*
  • Optics and Photonics / instrumentation*
  • Prostatic Neoplasms / pathology
  • Rats
  • Sensitivity and Specificity
  • Silver
  • Spectrum Analysis, Raman / instrumentation
  • Spectrum Analysis, Raman / methods*
  • Surface Properties
  • Tumor Cells, Cultured

Substances

  • Gold Colloid
  • Silver
  • Indocyanine Green