Specific NF-kappa B subunits act in concert with Tat to stimulate human immunodeficiency virus type 1 transcription

J Virol. 1992 Jun;66(6):3883-7. doi: 10.1128/JVI.66.6.3883-3887.1992.

Abstract

NF-kappa B is a protein complex which functions in concert with the tat-I gene product to stimulate human immunodeficiency virus (HIV) transcription. To determine whether specific members of the NF-kappa B family contribute to this effect, we have examined the abilities of different NF-kappa B subunits to act with Tat-I to stimulate transcription of HIV in Jurkat T-leukemia cells. We have found that the p49(100) DNA binding subunit, together with p65, can act in concert with Tat-I to stimulate the expression of HIV-CAT plasmid. Little effect was observed with 50-kDa forms of p105 NF-kappa B or rel, in combination with p65 or full-length c-rel, which do not stimulate the HIV enhancer in these cells. These findings suggest that the combination of p49(100) and p65 NF-kappa B can act in concert with the tat-I gene product to stimulate the synthesis of HIV RNA.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cells, Cultured
  • Chloramphenicol O-Acetyltransferase / biosynthesis
  • Chloramphenicol O-Acetyltransferase / genetics
  • Gene Products, tat / metabolism*
  • HIV-1 / metabolism*
  • Humans
  • NF-kappa B / classification
  • NF-kappa B / metabolism*
  • Oncogene Proteins v-rel
  • Retroviridae Proteins, Oncogenic / metabolism
  • T-Lymphocytes / metabolism*
  • Transcription, Genetic*
  • Transcriptional Activation
  • Transfection
  • tat Gene Products, Human Immunodeficiency Virus

Substances

  • Gene Products, tat
  • NF-kappa B
  • Oncogene Proteins v-rel
  • Retroviridae Proteins, Oncogenic
  • tat Gene Products, Human Immunodeficiency Virus
  • Chloramphenicol O-Acetyltransferase