Microarray-based genotyping for blood groups: comparison of gene array and 5'-nuclease assay techniques with human platelet antigen as a model

Transfusion. 2005 May;45(5):654-9. doi: 10.1111/j.1537-2995.2005.04318.x.

Abstract

Background: Most blood group alloantigens specific for red cells and platelets (PLTs) are based on single-nucleotide polymorphisms (SNPs) in genes encoding relevant membrane proteins.

Study design and methods: By use of five human PLT antigen (HPA) systems as a model, the suitability of a fourth-generation microarray technique for SNP typing was investigated. The results of the former were compared with those of a parallel developed third-generation technique (TaqMan assay, Applied Biosystems). Both techniques were validated by use of a unique panel of 71 blinded DNA samples containing at least 15 aa, bb, and ab genotypes for the HPA-1, -2, -3, -5, and-15 systems.

Results: Unambiguous and concordant results were obtained with both techniques for all samples.

Conclusion: The data presented here validate the use of microarray for large-scale SNP typing for clinically relevant blood group alloantigens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Human Platelet / genetics*
  • Blood Grouping and Crossmatching / methods*
  • Genotype
  • Histocompatibility Testing / methods
  • Humans
  • Isoantigens / genetics
  • Microarray Analysis / methods*
  • Oligonucleotide Probes
  • Polymorphism, Single Nucleotide
  • Taq Polymerase

Substances

  • Antigens, Human Platelet
  • Isoantigens
  • Oligonucleotide Probes
  • Taq Polymerase