T-cell activation and memory phenotypes in cerebrospinal fluid during HIV infection

J Acquir Immune Defic Syndr. 2005 May 1;39(1):16-22. doi: 10.1097/01.qai.0000155036.03004.a0.

Abstract

We characterized T cell phenotypes in 74 paired blood and cerebrospinal fluid (CSF) samples of HIV-infected and uninfected persons using four-color flow cytometry. CD4+ and CD8+ T cells subsets were further characterized by identifying activated/resting and memory/naive subsets in CSF and blood using the markers CD38/HLA-DR and CD45RA/CD62L, respectively. With and without HIV-infection, the proportion of CD4+ T cells and memory T cells among T cells in CSF was higher compared to blood. In HIV-infection, activated CD4+ and CD8+ T cells in CSF were more abundant than in uninfected controls. As expected, combination antiretroviral therapy (ART) reduced T cell activation in CSF and blood.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Antiretroviral Therapy, Highly Active
  • CD4 Lymphocyte Count
  • CD8-Positive T-Lymphocytes / immunology*
  • Female
  • Flow Cytometry
  • HIV Infections / cerebrospinal fluid*
  • HIV Infections / immunology*
  • Humans
  • Immunologic Memory / genetics*
  • Lymphocyte Activation
  • Lymphocyte Count
  • Male
  • Middle Aged
  • Phenotype
  • RNA, Viral / blood
  • RNA, Viral / cerebrospinal fluid
  • Reference Values
  • T-Lymphocytes / immunology*
  • Viral Load

Substances

  • RNA, Viral