Assignment of the gene for porcine insulin-like growth factor binding protein 1 to chromosome 18 and detection of polymorphisms in intron 2 by PCR-RFLP

Yuko Inage-Miyake; Shin-ichi Shimanuki; Tomohito Itoh; Yasuto Murakami; Masahiro Kimura; Hideaki Suzuki; Masashi Miyake; Daisuke Toki; Hirohide Uenishi; Takashi Awata; Noriyuki Hamasima

doi:10.1007/s10528-005-1069-1

Assignment of the gene for porcine insulin-like growth factor binding protein 1 to chromosome 18 and detection of polymorphisms in intron 2 by PCR-RFLP

Biochem Genet. 2005 Feb;43(1-2):79-85. doi: 10.1007/s10528-005-1069-1.

Authors

Yuko Inage-Miyake¹, Shin-ichi Shimanuki, Tomohito Itoh, Yasuto Murakami, Masahiro Kimura, Hideaki Suzuki, Masashi Miyake, Daisuke Toki, Hirohide Uenishi, Takashi Awata, Noriyuki Hamasima

Affiliation

¹ Society for Techno-innovation of Agriculture, Forestry and Fisheries Institute, Kamiyokoba, Tsukuba, Ibaraki 305-0854, Ibaraki, Japan.

PMID: 15859522
DOI: 10.1007/s10528-005-1069-1

Abstract

We have obtained a partial cDNA and three BAC clones for the porcine insulin-like growth factor binding protein 1 gene (IGFBP-1). Results of fluorescence in situ and radiation hybrid (RH) mapping assigned this gene to porcine chromosome (SSC) 18q24-qter. We found two types of polymerase chain reaction-restriction-fragment-length polymorphisms (PCR-RFLP) in intron 2 by using FokI and AluI.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chromosome Mapping
In Situ Hybridization, Fluorescence
Insulin-Like Growth Factor Binding Protein 1 / genetics*
Introns / genetics
Polymerase Chain Reaction
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
Swine / genetics*

Substances

Insulin-Like Growth Factor Binding Protein 1