Localization of chaperones DnaK and GroEL in bacterial inclusion bodies

M Mar Carrió; Antonio Villaverde

doi:10.1128/JB.187.10.3599-3601.2005

Localization of chaperones DnaK and GroEL in bacterial inclusion bodies

J Bacteriol. 2005 May;187(10):3599-601. doi: 10.1128/JB.187.10.3599-3601.2005.

Authors

M Mar Carrió¹, Antonio Villaverde

Affiliation

¹ Institut de Biotecnologia i de Biomedicina and Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain

Abstract

By immunostaining and transmission electron microscopy, chaperones DnaK and GroEL have been identified at the solvent-exposed surface of bacterial inclusion bodies and entrapped within these aggregates, respectively. Functional implications of this distinct localization are discussed in the context of Escherichia coli protein quality control.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chaperonin 60 / metabolism*
Escherichia coli / metabolism*
Escherichia coli / ultrastructure
Escherichia coli Proteins / metabolism*
HSP70 Heat-Shock Proteins / metabolism*
Inclusion Bodies / metabolism*
Inclusion Bodies / ultrastructure
Microscopy, Immunoelectron

Substances

Chaperonin 60
Escherichia coli Proteins
HSP70 Heat-Shock Proteins
dnaK protein, E coli