Histological co-localization of iron in Abeta plaques of PS/APP transgenic mice

Neurochem Res. 2005 Feb;30(2):201-5. doi: 10.1007/s11064-004-2442-x.

Abstract

This study confirms the presence of iron, co-localized with Abeta plaques, in PS/APP mouse brain, using Perls' stain for Fe3+ supplemented by 3,3'-diaminobenzidine (DAB) and Abeta immunohistochemistry in histological brains sections fixed with formalin or methacarn. In this study, the fixation process and the slice thickness did not interfere with the Perls' technique. The presence of iron in beta-amyloid plaques in PS/APP transgenic mice, a model of Alzheimer's disease (AD) pathology, may explain previous reports of reductions of transverse relaxation time (T2) in MRI studies and represent the source of the intrinsic Abeta plaque MR contrast in this model.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3,3'-Diaminobenzidine
  • Alzheimer Disease / metabolism*
  • Alzheimer Disease / pathology*
  • Amyloid beta-Peptides / genetics
  • Amyloid beta-Peptides / metabolism*
  • Animals
  • Caudate Nucleus / pathology
  • Immunohistochemistry
  • Iron / metabolism*
  • Magnetic Resonance Imaging
  • Mice
  • Mice, Transgenic
  • Plaque, Amyloid / metabolism*
  • Plaque, Amyloid / pathology
  • Putamen / metabolism
  • Tissue Fixation

Substances

  • Amyloid beta-Peptides
  • 3,3'-Diaminobenzidine
  • Iron