Enhancing of measles virus infection by magnetofection

J Virol Methods. 2005 Sep;128(1-2):61-6. doi: 10.1016/j.jviromet.2005.04.003.

Abstract

Magnetofection is a viral and non-viral gene delivery method using polyethyleneimine-conjugated super-paramagnetic nanoparticle under a magnetic field. Previous studies have indicated that magnetofection enhanced the infection of adenoviruses and retroviruses. It is shown that magnetofection enhances the infection of measles virus, a paramyxovirus. When cells expressing a measles virus receptor human SLAM were infected with a measles virus that encodes green fluorescent protein gene, magnetofection enhanced measles virus infection by 30- to 70-fold. The infection of SLAM-negative cells with measles virus was also enhanced by magnetofection, but to a lesser extent. These results indicate that magnetofection could be useful for isolation of measles virus from clinical specimens.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD
  • CHO Cells / virology
  • Cell Line
  • Chlorocebus aethiops
  • Cricetinae
  • Glycoproteins / genetics
  • Glycoproteins / metabolism
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • HeLa Cells / virology
  • Humans
  • Immunoglobulins / genetics
  • Immunoglobulins / metabolism
  • Magnetics*
  • Measles virus / genetics
  • Measles virus / metabolism
  • Measles virus / pathogenicity*
  • Receptors, Cell Surface
  • Signaling Lymphocytic Activation Molecule Family Member 1
  • Vero Cells / virology

Substances

  • Antigens, CD
  • Glycoproteins
  • Immunoglobulins
  • Receptors, Cell Surface
  • Green Fluorescent Proteins
  • Signaling Lymphocytic Activation Molecule Family Member 1