Abstract
The insecticidal protoxin from Bacillus thuringiensis has been shown to be a major component of the spore coat. We have developed a novel surface display system using B. thuringiensis spores in which the N-terminal portion of the protoxin is replaced with a heterologous protein. The expression vector with a sporulation-specific promoter was successfully used to display green fluorescent protein and a single-chain antibody (scFv) gene that encodes anti-4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (anti-phOx) antibody. The spores that carry the anti-phOx antibody can bind to phOx specifically.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacillus thuringiensis / genetics
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Bacillus thuringiensis / metabolism*
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Bacillus thuringiensis / physiology
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Bacillus thuringiensis Toxins
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Bacterial Proteins / genetics*
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Bacterial Toxins / genetics*
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Electroporation
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Endotoxins / genetics*
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Gene Expression Regulation, Bacterial
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Genetic Vectors
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Green Fluorescent Proteins
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Hemolysin Proteins
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Immunoglobulin Variable Region / metabolism*
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Luminescent Proteins / metabolism*
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Oxazoles / immunology*
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Promoter Regions, Genetic
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Recombinant Fusion Proteins / metabolism*
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Single-Chain Antibodies
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Spores, Bacterial / genetics
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Spores, Bacterial / metabolism*
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Transformation, Bacterial
Substances
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4-ethoxymethylene-2-phenyl-2-oxazoline-5-one
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Bacillus thuringiensis Toxins
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Bacterial Proteins
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Bacterial Toxins
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Endotoxins
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Hemolysin Proteins
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Immunoglobulin Variable Region
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Luminescent Proteins
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Oxazoles
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Recombinant Fusion Proteins
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Single-Chain Antibodies
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insecticidal crystal protein, Bacillus Thuringiensis
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scFV green fluorescent protein, recombinant
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Green Fluorescent Proteins