Objective: To investigate the expression of JWA gene, heat shock proteins (hsp70 and hsp27) and p53, and to explore the role and the possible mechanism of JWA gene involved in H2O2-induced oxidative stress of K562 cells.
Methods: 0.01, 0.1, 1 mmol/L H2O2 treated K562 cells at 10, 30, 60 and 180 min to established the models of DNA damage. Furthermore, K562 cells were induced apoptosis by 0.1 mmol/L H2O2 at different time (6-48 h) and different concentration (0.5-1,000 micromol/L) of H2O2 at 48 h. DNA damage and cell apoptosis were detected by DNA gel electrophoresis. And the immunoblotting assay was used for detecting expressions of JWA protein and correlated genes (hsp27, hsp70 and p53).
Results: During the DNA damage, JWA was much more sensitive to H2O2 than those heat shock proteins, and its expression pattern was very similar to that of hsp70. And at low concentration of H2O2-exposure (0.01 mmol/L), the expressions of JWA and heat shock proteins were all increased greatly. In addition, JWA, hsp70, hsp27 and p53 overexpressed and their expression pattern were similar during cell apoptosis.
Conclusion: JWA should be functioning as an effective environmental responsive gene and should actively participate the signal pathways of oxidative stress which might be associated with hsp70 and p53.