Downregulation of vascular endothelial-cadherin expression is associated with an increase in vascular tumor growth and hemorrhagic complications

Thromb Haemost. 2005 Jun;93(6):1041-6. doi: 10.1160/TH04-10-0680.

Abstract

The pathogenesis of vascular tumors such as angiosarcomas is poorly understood. Cadherin expression inversely correlates with tumor malignancy and the endothelial specific VE-cadherin is low or absent in angiosarcomas, suggesting an inhibitory role for this protein in tumor progression. In this paper we report that PmyT VE-cadherin null (VEC null) endothelial cells form larger vascular tumors in nude mice when injected subcutaneously as compared to isogenic VE-cadherin positive (VEC pos) cells. This effect requires the association of beta-catenin to VEcadherin, since a VE-cadherin mutant lacking the domain responsible for beta-catenin binding (Deltabetacat) cannot rescue the phenotype. In VEC null cells beta-catenin is phosphorylated and partly degraded. N-cadherin is increased and detected at junctions. VEC null cells also present an altered fibrinolytic activity with increases in tPA, uPA, uPAR and a strong reduction in PAI-1, which may be correlated to the high incidence of abrupt hemorrhages in VEC null tumors. Overall, these data strongly suggest that downregulation of VE-cadherin in endothelial tumors may have important consequences for tumor growth and bleeding complications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD
  • Cadherins / genetics*
  • Cadherins / physiology
  • Cell Line
  • Down-Regulation
  • Endothelium, Vascular / physiopathology
  • Fibrinolysis
  • Hemangiosarcoma / etiology*
  • Hemangiosarcoma / genetics
  • Hemangiosarcoma / pathology
  • Hemangiosarcoma / physiopathology
  • Hemorrhage / etiology*
  • Humans
  • Male
  • Mice
  • Mice, Nude
  • Neoplasm Transplantation
  • Plasminogen Activator Inhibitor 1 / genetics
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism

Substances

  • Antigens, CD
  • Cadherins
  • Plasminogen Activator Inhibitor 1
  • RNA, Messenger
  • cadherin 5