X-ray spectroscopy of the Mn4Ca cluster in the water-oxidation complex of Photosystem II

Photosynth Res. 2005;85(1):73-86. doi: 10.1007/s11120-005-0638-9.

Abstract

The water-oxidation complex of Photosystem II (PS II) contains a heteronuclear cluster of 4 Mn atoms and a Ca atom. Ligands to the metal cluster involve bridging O atoms, and O and N atoms from amino acid side-chains of the D1 polypeptide of PS II, with likely additional contributions from water and CP43. Although moderate resolution X-ray diffraction-based structures of PS II have been reported recently, and the location of the Mn4Ca cluster has been identified, the structures are not resolved at the atomic level. X-ray absorption (XAS), emission (XES), resonant inelastic X-ray scattering (RIXS) and extended X-ray absorption fine structure (EXAFS) provide independent and potentially highly accurate sources of structural and oxidation-state information. When combined with polarized X-ray studies of oriented membranes or single-crystals of PS II, a more detailed picture of the cluster and its disposition in PS II is obtained.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Calcium Compounds / chemistry*
  • Calcium Compounds / metabolism
  • Manganese Compounds / chemistry*
  • Manganese Compounds / metabolism
  • Oxidation-Reduction
  • Photosystem II Protein Complex / chemistry*
  • Photosystem II Protein Complex / metabolism
  • Spectrometry, X-Ray Emission
  • Water / chemistry
  • Water / metabolism*

Substances

  • Calcium Compounds
  • Manganese Compounds
  • Photosystem II Protein Complex
  • Water