Handling of dioxin measurement data in the presence of non-detectable values: overview of available methods and their application in the Seveso chloracne study

Chemosphere. 2005 Aug;60(7):898-906. doi: 10.1016/j.chemosphere.2005.01.055. Epub 2005 Mar 2.

Abstract

Exposure measurements of concentrations that are non-detectable or near the detection limit (DL) are common in environmental research. Proper statistical treatment of non-detects is critical to avoid bias and unnecessary loss of information. In the present work, we present an overview of possible statistical strategies for handling non-detectable values, including deletion, simple substitution, distributional methods, and distribution-based imputation. Simple substitution methods (e.g., substituting 0, DL/2, DL/ radical2, or DL for the non-detects) are the most commonly applied, even though the EPA Guidance for Data Quality Assessment discouraged their use when the percentage of non-detects is >15%. Distribution-based multiple imputation methods, also known as robust or "fill-in" procedures, may produce dependable results even when 50-70% of the observations are non-detects and can be performed using commonly available statistical software. Any statistical analysis can be conducted on the imputed datasets. Results properly reflect the presence of non-detectable values and produce valid statistical inference. We describe the use of distribution-based multiple imputation in a recent investigation conducted on subjects from the Seveso population exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), in which 55.6% of plasma TCDD measurements were non-detects. We suggest that distribution-based multiple imputation be the preferred method to analyze environmental data when substantial proportions of observations are non-detects.

MeSH terms

  • Acne Vulgaris / chemically induced
  • Chemistry Techniques, Analytical / methods
  • Data Interpretation, Statistical
  • Environmental Monitoring / statistics & numerical data*
  • Humans
  • Italy
  • Linear Models
  • Polychlorinated Dibenzodioxins / blood*

Substances

  • Polychlorinated Dibenzodioxins