Dimethyl sulfone in human cerebrospinal fluid and blood plasma confirmed by one-dimensional (1)H and two-dimensional (1)H-(13)C NMR

NMR Biomed. 2005 Aug;18(5):331-6. doi: 10.1002/nbm.966.

Abstract

(1)H-NMR spectroscopy at 500 MHz was used to confirm that a previously unidentified singlet resonance at 3.14 ppm in the spectra of cerebrospinal fluid and plasma samples corresponds to dimethyl sulfone (DMSO(2)). A triple resonance inverse cryogenic NMR probe, with pre-amplifier and the RF-coils cooled to low temperature, was used to obtain an (1)H-(13)C HSQC spectrum of CSF containing 8 microM (753 ng/ml) DMSO(2). The (1)H-(13)C correlation signal for DMSO(2) was assigned by comparison with the spectrum from an authentic reference sample. In plasma and CSF from healthy controls, the concentration of DMSO(2) ranged between 0 and 25 micromol/l. The concentration of DMSO(2) in plasma from three of four patients with severe methionine adenosyltransferase I/III (MAT I/III) deficiency was about twice the maximum observed for controls. Thus, DMSO(2) occurs as a regular metabolite at low micromolar concentrations in cerebrospinal fluid and plasma. It derives from dietary sources, from intestinal bacterial metabolism and from human endogenous methanethiol metabolism.

Publication types

  • Clinical Trial
  • Comparative Study
  • Controlled Clinical Trial

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Biomarkers / cerebrospinal fluid
  • Biomarkers / metabolism
  • Carbon Isotopes
  • Child
  • Child, Preschool
  • Dimethyl Sulfoxide
  • Female
  • Humans
  • Infant
  • Infant, Newborn
  • Magnetic Resonance Spectroscopy / methods*
  • Male
  • Metabolic Diseases / blood*
  • Metabolic Diseases / cerebrospinal fluid*
  • Metabolic Diseases / diagnosis
  • Middle Aged
  • Nervous System Diseases / blood*
  • Nervous System Diseases / cerebrospinal fluid*
  • Nervous System Diseases / diagnosis
  • Protons
  • Sulfones / blood*
  • Sulfones / cerebrospinal fluid*

Substances

  • Biomarkers
  • Carbon Isotopes
  • Protons
  • Sulfones
  • dimethyl sulfone
  • Dimethyl Sulfoxide