High-yield solution-phase synthesis of di- and trinucleotide blocks assisted by polymer-supported reagents

Org Lett. 2005 Aug 4;7(16):3485-8. doi: 10.1021/ol0511777.

Abstract

A new solution-phase phosphoramidite approach is reported for oligonucleotide synthesis employing recyclable solid-supported reagents. It uses polyvinyl pyridinium tosylate as the activator of a nucleoside-3'-O-phosphoramidite in the coupling step with a 5'-OH nucleoside or dinucleotide. The resulting phosphite triester was either sulfurized or oxidized using polystyrene-bound trimethylammonium tetrathionate or periodiate. This method avoids complicated purification steps, as excess reagents are easily removed by filtration. [reaction: see text]

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Catalysis
  • Indicators and Reagents
  • Molecular Structure
  • Oligonucleotides / chemical synthesis*
  • Oligonucleotides / chemistry
  • Polymers / chemistry*

Substances

  • Indicators and Reagents
  • Oligonucleotides
  • Polymers