Analysis of human immunodeficiency virus type 1 LTR-LTR junctions in peripheral blood mononuclear cells of infected individuals

J Gen Virol. 1992 Jun:73 ( Pt 6):1537-41. doi: 10.1099/0022-1317-73-6-1537.

Abstract

Circularized DNA species containing two long terminal repeat circle junctions were analysed in peripheral blood mononuclear cells of human immunodeficiency virus type 1 (HIV-1)-infected individuals. The circle junction fragments found could be classified into four groups: fragments containing a normal circle junction, fragments with deletions at the circle junction, fragments containing the primer binding site inserted at the circle junction, and fragments containing insertions at the circle junction derived from other regions of the HIV-1 genome.

MeSH terms

  • Base Sequence
  • DNA, Circular / blood
  • DNA, Viral / blood*
  • HIV Infections / blood*
  • HIV Infections / microbiology
  • HIV Long Terminal Repeat*
  • HIV-1 / genetics
  • HIV-1 / isolation & purification*
  • Humans
  • Molecular Sequence Data
  • Monocytes / microbiology*
  • Polymerase Chain Reaction
  • Sequence Alignment

Substances

  • DNA, Circular
  • DNA, Viral