A sericin-derived peptide protects sf9 insect cells from death caused by acute serum deprivation

Biotechnol Lett. 2005 Jul;27(13):893-7. doi: 10.1007/s10529-005-7177-5.

Abstract

Sericin is the silk protein enveloping fibroin fibers in cocoons. Sericin hydrolysate protects cultured Sf9 insect cells from death caused by serum deprivation; the activity depends on the repeats of 38 amino acids. A partial peptide from the 38 residues, SGGSSTYGYS, inhibited serum-deprivation death as well. Cell viabilities in the presence of 10% (v/v) foetal calf serum, no additives and 1 mM: SGGSSTYGYS were 96, 12 and 31% on the third day after inoculation, respectively. Aromatic residues seemed to be important because SGGSSTWGWS had the same activity as SGGSSTYGYS but SGGSSTAGAS had no activity.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Line
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Culture Media, Serum-Free / pharmacology
  • Dose-Response Relationship, Drug
  • Molecular Sequence Data
  • Peptide Fragments / pharmacology*
  • Sericins / chemistry
  • Sericins / pharmacology*
  • Spodoptera / cytology
  • Spodoptera / drug effects*
  • Time Factors

Substances

  • Culture Media, Serum-Free
  • Peptide Fragments
  • Sericins