Developing liquid chromatography ion mobility mass spectometry techniques

Expert Rev Proteomics. 2005 Aug;2(4):553-65. doi: 10.1586/14789450.2.4.553.

Abstract

When a packet of ions in a buffer gas is exposed to a weak electric field, the ions will separate according to differences in their mobilities through the gas. This separation forms the basis of the analytical method known as ion mobility spectroscopy and is highly efficient, in that it can be carried out in a very short time frame (micro- to milliseconds). Recently, efforts have been made to couple the approach with liquid-phase separations and mass spectrometry in order to create a high-throughput and high-coverage approach for analyzing complex mixtures. This article reviews recent work to develop this approach for proteomics analyses. The instrumentation is described briefly. Several multidimensional data sets obtained upon analyzing complex mixtures are shown in order to illustrate the approach as well as provide a view of the limitations and required future work.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Chromatography, Liquid / instrumentation*
  • Chromatography, Liquid / methods*
  • Computational Biology
  • Humans
  • Ions / chemistry
  • Mass Spectrometry / instrumentation*
  • Mass Spectrometry / methods*
  • Proteins / chemistry
  • Proteins / metabolism
  • Proteomics

Substances

  • Ions
  • Proteins