Use of non-aqueous capillary electrophoresis for the quality control of commercial saffron samples

J Chromatogr A. 2005 Sep 2;1085(2):293-8. doi: 10.1016/j.chroma.2005.06.041.

Abstract

A non-aqueous capillary electrophoresis (NACE) method for quantifying the seven crocin metabolites that are the major biologically active ingredients of saffron was developed. Separation is done by using a fused silica capillary filled with a 12.5 mM H3BO3/37.5 mM sodium tetraborate methanolic solution as background electrolyte. The results obtained were compared with the total index "safranal value", widely used as a quality measure of saffron products. The comparison revealed that the proposed NACE method provides useful information not obtained in the safranal value. Infact, samples with a similar safranal value can contain crocin metabolites in different concentrations and relative proportions. This new method is very useful for quality control in commercial saffron samples.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carotenoids / analysis*
  • Carotenoids / chemistry
  • Carotenoids / standards
  • Crocus / chemistry*
  • Electrophoresis, Capillary / methods*
  • Electrophoresis, Capillary / standards
  • Molecular Structure
  • Plant Preparations / analysis
  • Plant Preparations / chemistry
  • Quality Control

Substances

  • Plant Preparations
  • Carotenoids
  • crocin