Depletion of CD8+ or CD4+ lymphocytes enhances susceptibility to transplantable ultraviolet radiation-induced skin tumours

Anticancer Res. 2005 May-Jun;25(3B):1963-7.

Abstract

Background: Immunosuppressed patients are extremely susceptible to cutaneous squamous cell carcinoma, suggesting that immunosurveillance by T lymphocytes protects against this ultraviolet radiation-induced tumour.

Materials and methods: To determine the relative contribution of CD8+ and CD4+ lymphocytes to immunosurveillance, we tested the effects of CD8+ or CD4+ T lymphocyte depletion on the susceptibility of C3H/HeN mice to a syngeneic UVR-induced skin tumour cell line.

Results: Both anti-CD8 and anti-CD4 treatment significantly enhanced the growth of transplanted tumours. In CD8-depleted animals, tumours grew rapidly in all animals. Tumour growth in CD4-depleted animals was slower, and 50% of these mice eventually rejected their tumours. In CD4-depleted mice that did not reject their tumours, an early period of tumour growth was followed by partial regression of the tumour; a second phase of rapid tumour growth then supervened.

Conclusion: Our findings suggest that both CD8+ and CD4+ lymphocytes contribute to immunosurveillance against skin cancer.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • CD4 Lymphocyte Count
  • CD4-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / immunology*
  • Cell Line, Tumor
  • Immunologic Surveillance / immunology
  • Mice
  • Mice, Inbred C3H
  • Neoplasm Transplantation / immunology
  • Neoplasms, Radiation-Induced / etiology
  • Neoplasms, Radiation-Induced / immunology*
  • Neoplasms, Radiation-Induced / pathology
  • Skin Neoplasms / etiology
  • Skin Neoplasms / immunology*
  • Skin Neoplasms / pathology
  • Ultraviolet Rays