Thalassobacillus devorans gen. nov., sp. nov., a moderately halophilic, phenol-degrading, Gram-positive bacterium

Int J Syst Evol Microbiol. 2005 Sep;55(Pt 5):1789-1795. doi: 10.1099/ijs.0.63560-0.

Abstract

A novel moderately halophilic bacterium, strain G-19.1(T), has been isolated from a phenol enrichment of samples collected in hypersaline habitats of southern Spain. This enrichment culture was a part of a screening programme to isolate halophilic bacteria able to degrade various aromatic compounds. Strain G-19.1(T) has been characterized as a potential phenol-degrader over a wide range of saline conditions. Strain G-19.1(T) was found to be an aerobic, Gram-positive, endospore-forming, non-pigmented, moderately halophilic rod that grew optimally in media containing 7.5-10% NaCl at pH 7.0. The DNA G+C content was 42.4 mol%. Phylogenetic analysis based on comparison of 16S rRNA gene sequences indicated that the closest relatives were Halobacillus species (96.2-97.0%), although this novel isolate constitutes a separate line of descent within the radiation of Gram-positive rods. The cell-wall peptidoglycan contained meso-diaminopimelic acid, indicating that this strain does not share the main characteristic that differentiates members of the genus Halobacillus (which contain Orn-D-Asp) from other related genera. The predominant cellular fatty acids were anteiso-C(15:0), iso-C(16:0) and iso-C(15:0). On the basis of phenotypic, genotypic and phylogenetic analyses, this isolate should be classified in a novel genus and species, for which the name Thalassobacillus devorans gen. nov., sp. nov. is proposed. The type strain is strain G-19.1(T) (=DSM 16966(T)=CECT 7046(T)=CCM 7282(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillaceae / classification*
  • Bacillaceae / genetics*
  • Bacillaceae / growth & development
  • Bacillaceae / isolation & purification
  • Bacterial Typing Techniques
  • Base Composition
  • Biodegradation, Environmental
  • DNA, Bacterial
  • Environment*
  • Genes, rRNA
  • Genotype
  • Molecular Sequence Data
  • Phenol / metabolism*
  • Phenotype
  • Phylogeny
  • RNA, Ribosomal, 16S
  • Sodium Chloride*
  • Spain

Substances

  • DNA, Bacterial
  • RNA, Ribosomal, 16S
  • Phenol
  • Sodium Chloride

Associated data

  • GENBANK/AJ717299