Uptake and intracellular transport of cationic ferritin in the bronchiolar and alveolar epithelia of the rat

Cell Tissue Res. 1992 May;268(2):335-40. doi: 10.1007/BF00318802.

Abstract

Cationic ferritin was used as a marker to reveal the processes of endocytosis and intracellular transport in bronchiolar and alveolar epithelia. The marker was injected into the lung via the trachea, and ultrastructural observation of the distribution of ferritin particles in bronchiolar and alveolar epithelial cells was carried out at intervals of 5, 15, 30 and 60 min after the injection. The luminal surface of the airway and the alveolar epithelium showed diffuse labeling with cationic ferritin. In general, ferritin particles were observed in vesicles and vacuoles of the bronchiolar and alveolar epithelial cells within 5 min of injection; they appeared in multivesicular bodies within 15 min. Multivesicular bodies and secondary lysosomes containing ferritin particles, some of which showed a positive reaction for acid phosphatase, were seen in the basal cytoplasm within 30 min; ferritin particles appeared in the basal lamina below the Clara cells, ciliated cells and type 2 alveolar cells within 30 min. Ferritin particles were seen in ovoid granules of some Clara cells and in lamellar inclusion bodies of many type 2 alveolar cells. Brush cells and type 1 alveolar cells took up only a small quantity of ferritin particles.

MeSH terms

  • Animals
  • Bronchi / chemistry
  • Bronchi / metabolism*
  • Cations / metabolism
  • Endocytosis
  • Epithelium / chemistry
  • Epithelium / metabolism
  • Ferritins / metabolism*
  • Male
  • Microscopy, Electron
  • Pulmonary Alveoli / chemistry
  • Pulmonary Alveoli / metabolism*
  • Rats
  • Rats, Inbred Strains

Substances

  • Cations
  • Ferritins