The first crystal structure of a Mimosoideae lectin reveals a novel quaternary arrangement of a widespread domain

J Mol Biol. 2005 Oct 28;353(3):574-83. doi: 10.1016/j.jmb.2005.08.055. Epub 2005 Sep 9.

Abstract

The crystal structures of the apo and mannose-bound Parkia platycephala seed lectin represent the first structure of a Mimosoideae lectin and a novel circular arrangement of beta-prism domains, and highlight the adaptability of the beta-prism fold as a building block in the evolution of plant lectins. The P.platycephala lectin is a dimer both in solution and in the crystals. Mannose binding to each of the three homologous carbohydrate-recognition domains of the lectin occurs through different modes, and restrains the flexibility of surface-exposed loops and residues involved in carbohydrate recognition. The planar array of carbohydrate-binding sites on the rim of the toroid-shaped structure of the P.platycephala lectin dimer immediately suggests a mechanism to promote multivalent interactions leading to cross-linking of carbohydrate ligands as part of the host strategy against phytopredators and pathogens. The cyclic structure of the P.platycephala lectin points to the convergent evolution of a structural principle for the construction of lectins involved in host defense or in attacking other organisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Binding Sites
  • Crystallography, X-Ray
  • Lectins / chemistry*
  • Lectins / metabolism
  • Mannose / metabolism
  • Mimosa / chemistry*
  • Molecular Sequence Data
  • Protein Structure, Quaternary
  • Sequence Homology, Amino Acid

Substances

  • Lectins
  • Mannose

Associated data

  • PDB/1ZGR
  • PDB/1ZGS