Novel 13C direct detection experiments, including extension to the third dimension, to perform the complete assignment of proteins

J Magn Reson. 2006 Jan;178(1):56-64. doi: 10.1016/j.jmr.2005.08.011. Epub 2005 Sep 30.

Abstract

Carbon-13 direct detection NMR methods are feasible thanks to the improvements in probehead technology and to the development of new NMR experiments. We present here a complete set of experiments, based on C' direct detection, developed to perform protein complete assignment of backbone and side-chains (except for aromatic rings). This strategy offers alternative solutions for demanding situations (paramagnetic and/or large molecules) and can be useful in general in conjunction with conventional experiments.

MeSH terms

  • Carbon Isotopes
  • Humans
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Protein Conformation
  • Superoxide Dismutase / chemistry*

Substances

  • Carbon Isotopes
  • Superoxide Dismutase