Tularemia in Denmark: identification of a Francisella tularensis subsp. holarctica strain by real-time PCR and high-resolution typing by multiple-locus variable-number tandem repeat analysis

J Clin Microbiol. 2005 Oct;43(10):5355-8. doi: 10.1128/JCM.43.10.5355-5358.2005.

Abstract

We report ulceroglandular tularemia affecting an 8-year-old boy and the first recovery of Francisella tularensis in Denmark. A novel real-time PCR assay was used to identify the strain as F. tularensis subsp. holarctica (type B). Multiple-locus variable-number tandem repeat analysis demonstrated a close genetic relationship to strains from Norway.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques*
  • Base Sequence
  • Child
  • Cluster Analysis
  • DNA, Bacterial / analysis*
  • Denmark
  • Francisella tularensis / classification*
  • Francisella tularensis / genetics
  • Francisella tularensis / isolation & purification
  • Humans
  • Male
  • Minisatellite Repeats / genetics*
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Tularemia / diagnosis*
  • Tularemia / microbiology

Substances

  • DNA, Bacterial