Expression of survivin and apoptotic biomarkers in benign prostatic hyperplasia

J Urol. 2005 Nov;174(5):2046-50. doi: 10.1097/01.ju.0000176459.79180.d1.

Abstract

Purpose: We assessed the differential expression of survivin and other apoptotic markers in stromal and epithelial compartments of benign prostatic hyperplasia (BPH) and normal prostate tissue.

Materials and methods: Immunohistochemical staining for survivin, and transforming growth factor-beta1 and its receptors was done in normal prostate and BPH areas from 114 consecutive patients who underwent radical prostatectomy. Moreover, staining for survivin, Bcl-2, Bax, caspase-3 and Ki-67 was performed in prostate specimens from 23 consecutive patients who underwent open prostatectomy and 4 young patients who underwent cystoprostatectomy and had a normal prostate.

Results: Survivin and Bcl-2 expression increased incrementally from normal prostate to epithelial BPH to stromal BPH. Caspase-3 expression was higher in BPH epithelium than in BPH stroma, which in turn was higher than that in normal prostate. Ki-67 was significantly over expressed in BPH stroma and epithelium. Survivin expression in BPH tissue correlated with International Prostate Symptom Score, quality of life, post-void residual urine volume, maximum urine flow rate and transforming growth factor-beta1 expression.

Conclusions: Survivin is over expressed in BPH and it correlates with BPH parameters. Increases in proliferation and inhibition of apoptosis have a role in BPH.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Apoptosis
  • Biomarkers / analysis
  • Biopsy, Needle
  • Case-Control Studies
  • Caspase 3
  • Caspases / analysis
  • Caspases / metabolism*
  • Humans
  • Immunohistochemistry
  • Inhibitor of Apoptosis Proteins
  • Male
  • Microtubule-Associated Proteins / analysis
  • Microtubule-Associated Proteins / metabolism*
  • Middle Aged
  • Neoplasm Proteins / analysis
  • Neoplasm Proteins / metabolism*
  • Prognosis
  • Prostatectomy / methods
  • Prostatic Hyperplasia / metabolism
  • Prostatic Hyperplasia / pathology*
  • Prostatic Hyperplasia / surgery
  • Proto-Oncogene Proteins c-bcl-2 / analysis
  • Proto-Oncogene Proteins c-bcl-2 / metabolism*
  • Reference Values
  • Risk Factors
  • Sensitivity and Specificity
  • Survivin

Substances

  • BIRC5 protein, human
  • Biomarkers
  • Inhibitor of Apoptosis Proteins
  • Microtubule-Associated Proteins
  • Neoplasm Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • Survivin
  • CASP3 protein, human
  • Caspase 3
  • Caspases