[Generation of chemical-inducible activation tagging T-DNA insertion lines of Arabidopsis thaliana]

Yi Chuan Xue Bao. 2005 Oct;32(10):1082-8.
[Article in Chinese]

Abstract

Using an estrogen-inducible expression XVE (LexA-VP16-Estragon Receptor) system, we have generated approximately 40 000 independent T-DNA insertion lines of Arabidopsis thaliana. Segregation analyses of about 18000 lines indicated that 51.6% of them contain single T-DNA insertions and that the average insertion number is 1.38 copies per line. Mutants displaying a variety of morphological alterations were identified, including those that affect development of roots,hypocotyls, leaves, floral organs and seeds as well as the flowering time.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / anatomy & histology
  • Arabidopsis / genetics*
  • Arabidopsis / growth & development
  • Bacterial Proteins / genetics
  • Cloning, Molecular
  • DNA, Bacterial / genetics*
  • Estrogens / pharmacology
  • Genetic Vectors / genetics
  • Herpes Simplex Virus Protein Vmw65 / genetics
  • Mutagenesis, Insertional / drug effects
  • Mutagenesis, Insertional / methods*
  • Phenotype
  • Plants, Genetically Modified / anatomy & histology
  • Plants, Genetically Modified / genetics*
  • Plants, Genetically Modified / growth & development
  • Plasmids / genetics
  • Receptors, Estrogen / genetics
  • Serine Endopeptidases / genetics
  • Transcriptional Activation / drug effects

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • Estrogens
  • Herpes Simplex Virus Protein Vmw65
  • LexA protein, Bacteria
  • Receptors, Estrogen
  • T-DNA
  • Serine Endopeptidases