Abstract
Using an estrogen-inducible expression XVE (LexA-VP16-Estragon Receptor) system, we have generated approximately 40 000 independent T-DNA insertion lines of Arabidopsis thaliana. Segregation analyses of about 18000 lines indicated that 51.6% of them contain single T-DNA insertions and that the average insertion number is 1.38 copies per line. Mutants displaying a variety of morphological alterations were identified, including those that affect development of roots,hypocotyls, leaves, floral organs and seeds as well as the flowering time.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis / anatomy & histology
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Arabidopsis / genetics*
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Arabidopsis / growth & development
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Bacterial Proteins / genetics
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Cloning, Molecular
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DNA, Bacterial / genetics*
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Estrogens / pharmacology
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Genetic Vectors / genetics
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Herpes Simplex Virus Protein Vmw65 / genetics
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Mutagenesis, Insertional / drug effects
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Mutagenesis, Insertional / methods*
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Phenotype
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Plants, Genetically Modified / anatomy & histology
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Plants, Genetically Modified / genetics*
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Plants, Genetically Modified / growth & development
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Plasmids / genetics
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Receptors, Estrogen / genetics
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Serine Endopeptidases / genetics
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Transcriptional Activation / drug effects
Substances
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Bacterial Proteins
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DNA, Bacterial
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Estrogens
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Herpes Simplex Virus Protein Vmw65
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LexA protein, Bacteria
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Receptors, Estrogen
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T-DNA
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Serine Endopeptidases