Abstract
To study the role of rab5a gene in the mechanism of tumor metastasis, rab5a gene was stable transfected into AGZY83-a cell, a lung adenocarcinoma cell line with low metastatic potential. Superarray was applied to study the effect of rab5a on the differential expression of genes related to tumor metastasis. Five differentially expressed genes were obtained, including one up-regulated gene s100a4 and 4 down-regulated genes, nm23a, rac1, cst3 and col4a2. These results were confirmed at both the RNA and protein levels. We concluded that rab5a promoted tumor metastasis by affecting multiple genes in several pathways involved in tumor metastasis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma / genetics
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Adenocarcinoma / metabolism
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Adenocarcinoma / pathology
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Blotting, Western
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Cell Line, Tumor
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Cystatin C / genetics
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Cystatin C / metabolism
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Gene Expression Regulation, Neoplastic
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Humans
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Lung Neoplasms / genetics
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Lung Neoplasms / metabolism
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Lung Neoplasms / pathology
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NM23 Nucleoside Diphosphate Kinases / genetics
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NM23 Nucleoside Diphosphate Kinases / metabolism
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Neoplasm Metastasis / genetics
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Neoplasm Metastasis / physiopathology*
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Nucleic Acid Hybridization
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RNA, Neoplasm / genetics
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RNA, Neoplasm / metabolism
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S100 Calcium-Binding Protein A4
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S100 Proteins / genetics
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S100 Proteins / metabolism
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Transfection
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rab5 GTP-Binding Proteins / genetics
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rab5 GTP-Binding Proteins / metabolism
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rab5 GTP-Binding Proteins / physiology*
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rac1 GTP-Binding Protein / genetics
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rac1 GTP-Binding Protein / metabolism
Substances
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CST3 protein, human
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Cystatin C
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NM23 Nucleoside Diphosphate Kinases
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RNA, Neoplasm
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S100 Calcium-Binding Protein A4
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S100 Proteins
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S100A4 protein, human
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NME1 protein, human
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rab5 GTP-Binding Proteins
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rac1 GTP-Binding Protein