Abstract
A real-time PCR assay targeting the highly specific erm34 sequence of Bacillus clausii DNA was developed and optimized. The quantitative assay showed a sensitivity level of 10(2) CFU/microl of sample. The method may represent a useful tool for monitoring the role of B. clausii as probiotic in vivo.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology
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Aztreonam / pharmacology
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Bacillus / genetics
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Bacillus / growth & development*
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Bacillus / isolation & purification*
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Bacterial Proteins / genetics
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Chloramphenicol / pharmacology
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Colony Count, Microbial
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Culture Media
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DNA, Bacterial / analysis
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DNA, Bacterial / isolation & purification
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Drug Resistance, Bacterial
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Feces / microbiology*
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Humans
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Methyltransferases / genetics*
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Polymerase Chain Reaction / methods*
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Probiotics
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Culture Media
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DNA, Bacterial
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Chloramphenicol
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Methyltransferases
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Aztreonam