Effects of a biodegradable polymer synthesized with inorganic tin on the chondrogenesis of human articular chondrocytes

J Biomed Mater Res A. 2006 Apr;77(1):84-9. doi: 10.1002/jbm.a.30616.

Abstract

Recent study has shown that biodegradable polymers are attractive candidates for chondrocyte fixation and further transplantation in cartilage tissue engineering. Poly (glycolic acid) (PGA), a polymer of glycolic acid, is widely used in orthopedic applications as a biodegradable polymer. Organotin, lead, antimony, and zinc are catalysts commonly used in synthesizing PGA. Here, we investigated the biocompatibility of PGA, synthesized with and without inorganic tin as a catalyst in chondrogenesis of human articular chondrocytes in a micromass culture system. Significant enhancement of chondrocyte proliferation and expression of the collagen type II protein gene were observed in cultures treated with PGA synthesized with a tin catalyst. However, aggrecan gene expression was very similar to the control culture. Amount of collagen type II protein was also increased in the same group of cultured chondrocytes. In contrast, PGA without a catalyst caused overall inhibition of chondrogenesis. Despite several positive findings, extensive investigations are essential for the feasibility of this PGA(Sn) in future clinical practice.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Absorbable Implants*
  • Biocompatible Materials* / chemistry
  • Cell Differentiation
  • Cell Proliferation
  • Cells, Cultured
  • Chondrocytes / cytology
  • Chondrocytes / metabolism*
  • Chondrogenesis / physiology*
  • Collagen Type II / genetics
  • Collagen Type II / metabolism
  • Extracellular Matrix / physiology
  • Gene Expression
  • Humans
  • Polyglycolic Acid* / chemical synthesis
  • Polyglycolic Acid* / chemistry
  • Polymers* / chemistry
  • Tin* / chemistry

Substances

  • Biocompatible Materials
  • Collagen Type II
  • Polymers
  • Polyglycolic Acid
  • Tin