Quantification of phagocytosis of Aspergillus conidia by macrophages using a novel antibody-independent assay

Kathrin Luther; Manfred Rohde; Jürgen Heesemann; Frank Ebel

doi:10.1016/j.mimet.2005.11.008

Quantification of phagocytosis of Aspergillus conidia by macrophages using a novel antibody-independent assay

J Microbiol Methods. 2006 Jul;66(1):170-3. doi: 10.1016/j.mimet.2005.11.008. Epub 2006 Jan 18.

Authors

Kathrin Luther¹, Manfred Rohde, Jürgen Heesemann, Frank Ebel

Affiliation

¹ Max-von-Pettenkofer-Institut, Ludwig-Maximilians-Universität, Pettenkofer-Strasse 9a, 80336 Munich, Germany.

PMID: 16412526
DOI: 10.1016/j.mimet.2005.11.008

Abstract

The pathogenic mould Aspergillus fumigatus can cause severe infections in immunocompromised patients. Phagocytosis of inhaled conidia is an early and crucial event in the defense of A. fumigatus infections. Here we describe a novel antibody-independent assay for quantification of phagocytosis, that in this study has been applied to different Aspergillus species, but that is in principle suitable for many fungi.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Aspergillosis / immunology*
Aspergillosis / microbiology
Aspergillus fumigatus / immunology*
Benzenesulfonates / chemistry
Biotin / chemistry
Cell Line
Cytochalasin D / pharmacology
Fluorescent Dyes / chemistry
Humans
Macrophages, Alveolar / immunology*
Macrophages, Alveolar / microbiology
Mice
Microscopy, Fluorescence
Phagocytosis / immunology

Substances

Benzenesulfonates
Fluorescent Dyes
Cytochalasin D
Biotin
C.I. Fluorescent Brightening Agent 28