Expression of APOBEC2 is transcriptionally regulated by NF-kappaB in human hepatocytes

FEBS Lett. 2006 Feb 6;580(3):731-5. doi: 10.1016/j.febslet.2005.12.081. Epub 2006 Jan 3.

Abstract

Apolipoprotein B mRNA-editing enzyme catalytic subunit 2 (APOBEC2) is a member of the nucleic-acid-editing enzymes. However, the physiological function of APOBEC2 remains unclear. We demonstrate that APOBEC2 expression is strongly enhanced in response to both tumour necrosis factor-alpha (TNF-alpha) and interleukin-1beta. Inhibition of NF-kappaB activation invariably blocks TNF-alpha-induced APOBEC2 expression. The promoter region of APOBEC2 contains functional NF-kappaB response elements in the 5' untranslated region of the gene at -625/-616. These results show that APOBEC2 expression is regulated by pro-inflammatory cytokines via NF-kappaB activation and suggest a possible role of APOBEC2 in the pathophysiology of hepatic inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • APOBEC Deaminases
  • Cell Line, Tumor
  • Cytidine Deaminase / biosynthesis*
  • Cytidine Deaminase / genetics
  • Gene Expression Regulation* / drug effects
  • Gene Expression Regulation* / genetics
  • Hepatitis / enzymology
  • Hepatitis / genetics
  • Hepatitis / pathology
  • Hepatocytes / enzymology*
  • Humans
  • Interleukin-1 / metabolism
  • Interleukin-1 / pharmacology
  • Muscle Proteins / biosynthesis*
  • Muscle Proteins / genetics
  • NF-kappa B / metabolism*
  • RNA Editing* / drug effects
  • RNA Editing* / genetics
  • Response Elements / genetics
  • Tumor Necrosis Factor-alpha / metabolism
  • Tumor Necrosis Factor-alpha / pharmacology

Substances

  • Interleukin-1
  • Muscle Proteins
  • NF-kappa B
  • Tumor Necrosis Factor-alpha
  • APOBEC Deaminases
  • APOBEC2 protein, human
  • Cytidine Deaminase