Some observations on the adaptation of Eimeria tenella (local isolates) sporozoites on chicken embryos through chorioallantoic membrane

J Vet Sci. 2006 Mar;7(1):59-61. doi: 10.4142/jvs.2006.7.1.59.

Abstract

Eimeria (E.) tenella (local isolate) sporozoites were adapted on the chorioallantoic membrane (CAM) of 10-12 days chicken embryos and completed its life cycle in 6-7 days at 39 degrees C and 70 per cent humidity. Only 23 embryos (4.6%) were found dead from 1-4 day post inoculation of sporozoites with mild lesions on CAM with no gametocytes but few sporozoites in chorioallantoic fluid (CAF). On 5-7 day post inoculation, 432 embryos (86.4%) were found dead with severe haemorrhages on CAM and CAF contained uncountable number of gametocytes. After seven days post inoculation, 45 embryos (9%) were found to be alive. Some oocysts were also detected in the CAF on 6-7 days post inoculation. In the histological sections of the CAM, there were abundant small dark colored rounded bodies of gametes; distributed extensively in tissues of CAM on 5-7 days post inoculation of sporozoites. In some cases, cluster of small mature and immature relatively large bodies were seen in increasing numbers on 5-6 days post inoculation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chick Embryo
  • Chickens*
  • Chorioallantoic Membrane / parasitology*
  • Coccidiosis / parasitology
  • Coccidiosis / veterinary*
  • Eimeria tenella / growth & development*
  • Histocytochemistry
  • Poultry Diseases / parasitology*