Abstract
The hyperosmotic stress response of Streptococcus mutans was investigated. Real-time reverse transcriptase-PCR and slot-blot analysis revealed that opuAA, opcA, Smu.2115, sodA and nox were induced after exposure to 0.4 M NaCl. Our data suggest that there is a cross-talk between osmotic and oxidative stress responses in S. mutans. Inactivation of Smu.2115, encoding a putative oxidoreductase, resulted in an acid-resistant and hydrogen peroxide-sensitive phenotype.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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ATP-Binding Cassette Transporters / genetics
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ATP-Binding Cassette Transporters / metabolism
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Biofilms / growth & development
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Gene Deletion
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Gene Expression Regulation, Bacterial*
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Heat-Shock Response*
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Humans
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Osmotic Pressure
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Oxidative Stress
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Oxidoreductases / genetics
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Oxidoreductases / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Sodium Chloride / pharmacology*
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Streptococcus mutans / genetics
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Streptococcus mutans / growth & development
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Streptococcus mutans / metabolism
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Streptococcus mutans / physiology*
Substances
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ATP-Binding Cassette Transporters
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Bacterial Proteins
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Sodium Chloride
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Oxidoreductases