Inflammation-induced changes in expression and glycosylation of genetic variants of alpha 1-acid glycoprotein. Studies with human sera, primary cultures of human hepatocytes and transgenic mice

Biochem J. 1991 Jun 1;276 ( Pt 2)(Pt 2):343-7. doi: 10.1042/bj2760343.

Abstract

The relative occurrence of genetic variants of human alpha 1-acid glycoprotein (AGP) in relation to changes in glycosylation was studied in sera of patients with burn injury, media of cytokine-treated primary cultures of human hepatocytes and Hep 3B cells, and sera of transgenic mice expressing the human AGP-A gene. It is concluded (i) that the glycosylation of AGP was not dependent on its genetic expression and (ii) that both the variants determined by the AGP-A gene as well as by the AGP-B/B' genes are increased after inflammation or treatment with interleukins 1 and 6.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Burns / blood
  • Burns / physiopathology
  • Carcinoma, Hepatocellular
  • Cells, Cultured
  • Cytokines / pharmacology
  • Gene Expression
  • Genetic Variation*
  • Glycosylation
  • Humans
  • Inflammation
  • Liver / drug effects
  • Liver / metabolism*
  • Liver Neoplasms
  • Mice
  • Mice, Transgenic
  • Orosomucoid / analysis
  • Orosomucoid / biosynthesis
  • Orosomucoid / genetics*

Substances

  • Cytokines
  • Orosomucoid