An animal model of capsular infarct: endothelin-1 injections in the rat

Behav Brain Res. 2006 May 15;169(2):206-11. doi: 10.1016/j.bbr.2006.01.014. Epub 2006 Feb 23.

Abstract

In this study stereotaxic injections of the vasoconstrictive peptide endothelin-1 (ET-1) were used to create infarcts in the white matter of the internal capsule underlying sensorimotor cortex in rats. Resulting deficits were assessed using established sensorimotor tests conducted on each rat before and after the ET-1-induced infarct. After a 14-day survival period, histological examination revealed tissue necrosis and demyelination in the infarcted white matter of ET-1-injected rats, but not saline-injected control rats. Infarcts resulted in measurable sensorimotor deficits in rats that received ET-1 injections. The same sensorimotor tests showed no deficits in surgical-control rats. The present model of white matter infarct should be valuable in examining the underlying mechanisms of subcortical ischemic stroke and to evaluate potential therapeutic interventions.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Behavior, Animal / drug effects
  • Brain Infarction / chemically induced*
  • Demyelinating Diseases / chemically induced
  • Demyelinating Diseases / pathology
  • Disease Models, Animal
  • Endothelin-1 / toxicity*
  • Forelimb / drug effects
  • Forelimb / physiopathology
  • Internal Capsule / drug effects*
  • Internal Capsule / pathology*
  • Male
  • Necrosis / chemically induced
  • Necrosis / pathology
  • Psychomotor Performance / drug effects
  • Rats
  • Rats, Sprague-Dawley
  • Vibrissae / drug effects
  • Vibrissae / innervation

Substances

  • Endothelin-1