Abstract
IL-18 is a multifunctional cytokine that augments both innate and acquired immunity and potentiates Th1 and Th2 reactions. We studied the expression of IL-18 receptor (IL-18R) on renal and respiratory epithelial cell lines. Both cell lines upregulated IL-18R mRNA and IL-18R membrane expression in response to TNF alpha and other proinflammatory cytokines. The function of IL-18R was confirmed by induction of IL-8 release from epithelial cells in response to recombinant IL-18. Epithelial cells may represent an important target for IL-18, mainly under inflammatory conditions associated with TNF alpha release.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Line, Tumor
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Enzyme-Linked Immunosorbent Assay
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Epithelial Cells / drug effects*
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Flow Cytometry
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Humans
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Interleukin-18 / genetics
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Interleukin-18 / pharmacology
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Interleukin-18 Receptor alpha Subunit
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Interleukin-8 / metabolism
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Receptors, Interleukin / metabolism*
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Receptors, Interleukin-18
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Recombinant Proteins / pharmacology
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Reverse Transcriptase Polymerase Chain Reaction
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Tumor Necrosis Factor-alpha / pharmacology*
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Up-Regulation / drug effects*
Substances
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IL18R1 protein, human
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Interleukin-18
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Interleukin-18 Receptor alpha Subunit
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Interleukin-8
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Receptors, Interleukin
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Receptors, Interleukin-18
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Recombinant Proteins
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Tumor Necrosis Factor-alpha