Crystallization and preliminary X-ray diffraction analysis of a lectin from Canavalia maritima seeds

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt 1):87-9. doi: 10.1107/S1744309104029197. Epub 2004 Dec 2.

Abstract

A lectin from Canavalia maritima seeds (ConM) was purified and submitted to crystallization experiments. The best crystals were obtained using the vapour-diffusion method at a constant temperature of 293 K and grew in 7 d. A complete structural data set was collected to 2.1 A resolution using a synchrotron-radiation source. The ConM crystal belongs to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 67.15, b = 70.90, c = 97.37 A. A molecular-replacement search found a solution with a correlation coefficient of 69.2% and an R factor of 42.5%. Crystallographic refinement is under way.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Canavalia / chemistry*
  • Crystallization
  • Plant Lectins / chemistry*
  • Plant Lectins / isolation & purification
  • Seeds / chemistry*
  • X-Ray Diffraction

Substances

  • Plant Lectins
  • lectin, Canavalia