Abstract
In this study, we have cloned the Chlamydia trachomatis genes incB and incC into the expression plasmid vectors from pET series for the subsequent isolation of recombinant proteins. As a result, we have obtained the first full-length recombinant C. trachomatis proteins IncB and IncC, which can be used for following antibody production and for study of their protein-protein interaction.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Bacterial Proteins / genetics
-
Bacterial Proteins / metabolism*
-
Chlamydia trachomatis
-
Escherichia coli / genetics
-
Escherichia coli / metabolism*
-
Female
-
Gene Expression Regulation, Bacterial
-
Humans
-
Membrane Proteins / genetics
-
Membrane Proteins / metabolism*
-
Molecular Sequence Data
-
Peptide Fragments / genetics
-
Peptide Fragments / metabolism
-
Polymorphism, Genetic
-
Recombinant Fusion Proteins / genetics
-
Recombinant Fusion Proteins / metabolism
-
Thioredoxins / genetics
-
Thioredoxins / metabolism
Substances
-
Bacterial Proteins
-
Membrane Proteins
-
Peptide Fragments
-
Recombinant Fusion Proteins
-
Thioredoxins